Analysis of subthreshold pace-maker currents in chick embryonic heart cells.
نویسندگان
چکیده
منابع مشابه
The Effect of chick Embryonic Somites on Neural Rosette Formation in Mouse Embryonic Stem Cells
purpose: The aim of the present study is to understand if EBs can generate neural rosette upon co-culture with chick embryo somites. Materials and Methods: The mouse ES cells, line Royan Bl, were cultured in hanging drops to induce embryoid bodies (EBs) formation. Somites were isolated from the chick embryos and then embedded in alginate solution. Finally, alginate beads containing somites were...
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We have developed a system for the co-culture of embryonic chick heart cells obtained from embryos at 3.5 days in ovo with ciliary ganglia from chick embryos at 7 days in vivo. After 3 days of co-culture, removal of the ciliary ganglia resulted in complete degeneration of axons within 6-8 h, leaving the post-innervated heart cell culture devoid of neurons. Embryonic chick heart cells at 3.5 day...
متن کاملEvaluation of effect of chick embryonic notochord on neural induction of mouse embryonic stem cells
Introduction: The aim of this study was evaluate the ability of notochord to induce neural induction and/or differentiation of mouse embryonic stem cell to neuron and motor neuron, respectively. Methods: In order to produce embryoid bodies, ES cells line Royan B1 were grown in suspension in the absence of LIF for 4 days. EBs were divided into 4 groups. EBs in group 1 & 2 were further cultur...
متن کاملCharacterization of outward potassium current in embryonic chick heart cells.
AIM To characterize a voltage-dependent outward K+ current in cultured heart cells of 14-16-day-old embryos of yellow chick. METHODS The patchclamp technique in the whole-cell configuration was used. RESULTS The kinetics and the pharmacology of the outward K+ current in our cell mold were different from those described in white chick. Like the calcium-activated K+ current, blocker of calciu...
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Spontaneous beating of heart-cell aggregates from 4-day chick embryos was initially blocked by 10(-5) g/ml tetrodotoxin (TTX). With continued exposure to the drug, the fraction of blocked aggregates decreased from about 80% at 15 min to about 25% at 2-3 h, at which time, beating aggregates had become desensitized to the toxin, showing no response to a fresh dose. Aggregates from 5-day hearts we...
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ژورنال
عنوان ژورنال: The Journal of Physiology
سال: 1981
ISSN: 0022-3751
DOI: 10.1113/jphysiol.1981.sp013639